Pembuatan Nutrisi dan Penyuluhan Penyakit Hipertensi pada Anggota PKK Delima Jakarta Timur

Menurut data SUDINKES DKI Jakarta tahun 2016 menunjukkan bahwa kotamadya Jakarta Timur memiliki
jumlah populasi yang hipertensi lebih banyak dibandingkan dengan daerah lainnnya yaitu sebanyak 98422
orang. Konsumsi buah dan sayuran segar akan memberikan banyak manfaat bagi tubuh kita, bukan saja
rasanya yang enak tapi buah dan sayur kaya akan antioksidan yang bermanfaat untuk mencegah terjadinya
stres oksidatif dan bisa menjadi salah satu upaya dalam mencegah dan mengobati hipertensi. Berbagai buah
dan sayuran banyak yang telah diteliti memberikan khasiat antihipertensi. Pada pengabdian masyarakat ini
kami mengadakan Pelatihan pembuatan nutrisi dan penyuluhan penyakit hipertensi pada anggota PKK RT.
014 Perumnas Klender Jakarta Timur. Buah-buahan dan sayur-sayuran yang kami gunakan adalah belimbing,
semangka, lemon, seledri dan mentimun.

Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.)

Soursop leaves (Annona muricata L.) is one of the medicinal plants identified as a source of endophytic
bacteria producing secondary metabolites. Several studies have reported that secondary metabolite
compounds extracted from soursop leaves have inhibitory activity against pathogenic bacteria and fungi and
have anticancer activity. This study aims to isolate endophytic bacteria of soursop leaf and identify
molecular isolates producing antibacterial metabolites by PCR method. This study began with endophytic
bacterial isolation of soursop leaves, followed by screening for antibacterial potency using disc diffusion
method and identification of molecular isolates which had the highest antibacterial activity. After isolation,
three isolates were obtained: BW-1LM, BW-2LP, and BW-3LK. The result of antibacterial activity test
showed that BW-1LM isolate had the highest activity against bacterium of Bacillus subtilis and Salmonella
typhi test. Molecular identification was obtained by BW-1LW isolate having 99% similarity level to
Bacillus licheniformis DSM 13 strain. The conclusion is soursop leaves contains endophytic bacteria which
have antibacterial activity against Bacillus subtilis and Salmonella typhi.

Quality Control of Turmeric Rhizome (Curcuma domestica Val) as Traditional Medicine from Wonogiri, Central Java

Turmeric is one of the plants that can be used as traditional medicine. To improve the quality of turmeric as
a traditional medicine, turmeric must be free from contamination of pesticide residues, aflatoxin, pathogen
bacteria, and curcumin content contained therein. The aim of this research was to investigate the
contamination of endosulfan and malathion pesticides, aflatoxin B1, Escherichia coli microbial
contamination, Salmonella sp., Staphylococcus aureus and Pseudomonas aeruginosa, as well as to know the
content of curcumin contained in turmeric rhizomes. The sample in this research was taken from Wonogiri
region of Central Java, Indonesia by random sampling. The methods used were HPLC for Aflatoxin B1
analysis and curcumin and Gas Chromatography for residual pesticide analysis of Endosulfan and
Malathion pesticides. Microbial testing included the establishment of Total Plate Count, AKK, MPN
Coliform, and analysis of Escherichia coli microbial contamination, Salmonella sp., Staphylococcus aureus
and Pseudomonas aeruginosa. The results showed that the samples were not contaminated by Aflatoxin B1
and Endosulfan pesticides, but contained a residual malathion with levels of 0.014 mg/kg. Microbial test
results showed that the turmeric samples from the Wonogiri market did not meet the quality requirements
due to contamination of Salmonella sp. and the chopped AKK exceeded the specified limits.

The Effect of Concentration Ratio of Gelatine and Polyvinylpyrrolidone as Binders on the Physical Properties of Red Ginger (Zingiber officinale Rosc.) Extract Lozenges

Lozenges require the tablet hardness of 7-14 Kgf to be a suitable binder. Gelatine can be used to meet the
requirement as it creates granules with bad flow time. PVP produces granules with better flow time but it
takes a large quantity to reach the lozenges hardness desired. This study aimed to determine the effect of the
concentration ratio of gelatine and PVP as binders on the physical properties of red-ginger extract lozenges.
Lozenges made employing the wet granulation method following the ratio of gelatine and PVP of 1:1, 1:2,
1:3, 1:4 and 1:5. The hardness test results of the formula 1 to 5 are 9, 14, 16, 13 and 12 Kgf respectively and
the friability test results were 0,4%, 0,7%, 0,2%, 0,3% and 0,6% accordingly. The combination of gelatine
and PVP as binders provide a significant difference in the hardness and friability of the tablets.

Antihyperglycemic Activity of Ethanolic Herb Extract of Ceplukan(Physalis angulata L.) in Diabetic Hypercholesterolemia in Male Hamsters

Diabetes mellitus is a disease characterized by hyperglycemia as well as progressive changes to the
pancreatic beta cell structure. This study was conducted to determine the antihyperglycemic activity of
ceplukan herb extract (Physalis angulata L.) in alloxan-induced male Syrian hamsters and high cholesterol
feed. The study used 24 hamsters divided into six groups. Group I were given a standard diet and regular
drinking water, Group II were given metformin dose 61,66 mg/kg body weight (BW), Group III were
alloxan-induced and high cholesterol feed, Groups IV, V and VI were given extract dose 60, 120 and 240
mg/kg BW respectively. The animals were induced alloxan monohydrate as well as were given high
cholesterol feed during treatment. Blood sampling was performed on the 29th and 44th day using a clinical
spectrophotometer. The results show significant differences between treatment groups (α <0,05), followed
by Tukey test. In conclusion, the preparation of herbal extract test ceplukan at doses of 120 and 240 mg/kg
BW could reduce blood glucose levels by 50.84% and 43.41% which is equivalent to metformin dose of
61.66 mg/kg BW with a percentage of 53.12%.

Pembuatan Obat Kumur Alami Daun Sirih Bagi Anggota Aisyiyah di PRA Cabang Perumnas I dan Jakasampurna

Daun sirih merupakan tanaman obat tradisional yang erat kaitannya dengan kesehatan gigi dan mulut. Daun
sirih diketahui sebagai salah satu Bahan alami yang memiliki kemampuan sebagai obat kumur karena
aktivitasnya sebagai antibakteri penyebab plak gigi. Meskipun telah banyak diketahui khasiatnya,
pemanfaatan dan pembuatan daun sirih sebagai obat kumur bagi masyarakat perlu disosialisasikan untuk
menjaga kesehatan mulut. Pengabdian masyarakat ini dilaksanakan dengan tujuan untuk meningkatkan
pengetahuan mengenai pencegahan bau mulut dengan obat kumur alami dan cara pembuatannya dengan
sederhana kepada masyarakat sehingga dapat meningkatkan derajat kesehatannya secara mandiri serta
meningkatkan pendapatan ekonomi. Mitra yang dipilih menjadi lokasi pengabdian masyarakat didasarkan
pada pertimbangan berdasarkan kepatutan sumber data dan informasi yang dikumpulkan melalui kunjungan
serta diskusi. Target yang dicapai adalah ibu-ibu rumah tangga yang tergolong masyarakat ekonomi
menengah ke bawah. Profil masyarakat di sebagian warga ini tepat untuk mendapatkan tambahan
pengetahuan dan keterampilan yang berguna untuk peningkatan kualitas hidup. Pengabdian masyarakat ini
dibagi menjadi 2 hari, hari pertama berupa pemaparan materi dan hari kedua berupa pelatihan pembuatan
obat kumur. Hasil yang diperoleh yaitu formula obat kumur yang sesuai dengan evaluasi rasa, warna, dan bau
dari orientasi formula. Formula yang terpilih yaitu menggunakan daun sirih dan daun mint (1:1). Dari
pelatihan ini diharapkan anggota Aisyiyah Cabang Perumnas I sebagai stake holder dapat menyebarkan
informasi resep pembuatan obat kumur yang sederhana dan harga terjangkau kepada anggota masyarakat
lainnya.

Construction of recombinant sox2-encoding plasmids that regulate pluripotency of breast cancer stem cells from indonesian patient

A therapy development with recombinant protein is a new and potential innovation to destroy
cancer stem cells (CSCs). Various ways of killing CSCs include the provision of polyvalent antiprotein
antibodies that code pluripotency. Therefore, it takes a mixture of Oct-4, c-Myc, Sox2, and
Klf4 protein antigens that can stimulate the formation of polyvalent antibodies. This study aimed to
construct Sox2 recombinant by identifying the target genes by reverse transcriptase PCR and then
arranging their designs to be inserted into the cloning vector pET101/D-TOPO®. The target gene was
developed by finding the complete sequences of Sox2 nucleotides on the NCBI GenBank. The growth
on LB-ampicillin agar plates was amplified by PCR to obtain colonies with pET101/D-TOPO®
vectors and inserts of the pluripotent gene of CSCs, then the PCR results were observed through
electrophoresis. A total of fifteen colonies have DNA bands with a base pair of about 300 bp in length.
The recombinant clones produced Sox2 genes with a base length of 330 bp.