Tag: ciracas.id

Development the Technique for the Preparation and Characterization of Reconstructed Human Epidermis (RHE)

Reconstructed Human Epidermis (RHE) is an artificial epidermis
made in such a way that it resembles human skin, and can be used for the
identification of irritant chemicals, especially for cosmetic and topical medicinal
products. Currently the new RHE is produced by European and American
countries, whose skin physiology is very different from Indonesia. Based on
this, the Center for Research on Drugs and Food, NADFC of Republic of
Indonesia took the initiative to develop the reconstruction of keratinocyte,
melanocytes and fibroblasts cells into RHE adapted to the anatomical and
physiological functions of the skin of Indonesians. RHE is made from an epidermal
layer composed of keratinocyte and melanocytes cells that are reconstructed
with a dermis layer composed of fibroblast and collagen cells.
Keratinocyte, melanocytes and fibroblasts cells are cultured on suitable mediums
by adding a suitable growth medium. To find out that RHE has been
successfully reconstructed, measured percentage of cell life, made histology
preparation to see the existence of cell nucleus, and conducted Immunohistochemical
examination to see existence of integration (bond) between antigen.
From the research results can be seen that keratinocyte cells grown on culture
medium Keratinocyte SFM (IX) with rEGF supplements; melanocyte cells
grown on Melanocyte 254 culture medium with HMGS supplementation; and
fibroblast cells grown on Fibroblast M 106 culture medium with LSGS supplementation.
The percentage of epidermal cell life grew well in the planting of
10 � 104 cells /mL keratinocytes and 0.25 � 104 cells /mL of melanocyte cells
and survived until the 11th day with live cell percentage of 93.45%. In making
preparation for histology with HE staining, there is a cell life in RHE tissue.
Used Immunohistochemical (IHC) examination using cytokeratin 10 antibody
marker to view physiological function of epidermal tissue.

Uji Larvasida Ekstrak n-heksan Daun Zodia (Evodia suaveolens Scheff.) Terhadap Dua Vektor Larva Nyamuk Aedes albopictus dan Culex spp

Insektisida nabati dapat digunakan untuk mengendalikan dan mencegah penyakit yang disebabkan oleh nyamuk. Salah satu alternatif bahan insektisida
nabati adalah daun zodia (Evodia suaveolens Scheff.) yang telah dikenal sebagai tanaman pengusir nyamuk. Penelitian ini bertujuan untuk mengetahui
efek larvasida dan nilai LC50 dari ekstrak daun zodia terhadap larva nyamuk Aedes albopictus dan Culex spp. Penelitian ini menggunakan ekstrak nheksan
daun zodia dengan variasi konsentrasi 10 ppm; 31,62 ppm; 99,98 ppm; 316,13 ppm; 1000 ppm dan kontrol. Metode yang digunakan adalah
metode bioassay yang telah distandarisasi oleh WHO (2005). Data hasil penelitian dianalisis menggunakan uji probit. Hasil penelitian menunjukkan
bahwa kematian 50% larva nyamuk Aedes albopictus dan Culex spp. (LC50-24 jam) adalah 106,036 ppm dan 116,9110 ppm. Berdasarkan hasil uji probit
nilai LC50 masuk ke dalam rentang 100-149 ppm maka dapat disimpulkan bahwa ekstrak n-heksan daun zodia termasuk ke dalam golongan larvasida
dengan tingkat toksisitas moderat tinggi.